味の素ジェネクシンのCHO細胞株用CELLiST™ BASAL培地は、味の素㈱の強みであるアミノ酸技術と、30年以上にわたる培地事業の経験をもとに開発されました。 CELLiST™培地は、動物由来成分を一切含まない、組成が既知(Chemically-defined)の高性能培地です。
BASAL CHO MX 培地は、アミノ酸とその他の栄養素を最適なバランスで配合することにより、培養プロセスにおいて、良好な細胞増殖と高い生産性を提供します。
また、BASAL CHO MX培地は、動物や植物由来の成分は一切含まない組成が既知(Chemically-defined)の培地です。
Model No. | BASAL CHO MX |
---|---|
TYPE | Powder |
Concentration | 23.0g / L |
With Additives | D-Glucose · Poloxamer |
Without Additives | L-Glutamine · Sodium hydrogen carbonate · Thymidine Hypoxanthine · Insulin and other growth factors |
Storage Condition | 2℃ to 8℃, dark and dry |
Shelf Life | 24 months |
Below are cell culture performance results for CELLiST BASAL CHO MX Medium and several other commer- cially-available media. The fed-batch process was performed using ambr15.
Figure 1. Viable cell density profiles during 14 days of culture.
Figure 2. IgG titer profile. IgG concentration was measured by Cedex Bio HT.
As can be seen below, CELLiST BASAL CHO MX Medium is suitable for use from small-scale to larger scale 200L bioreactors.
Figure 3. Viable cell density and IgG titer profiles. 3 kinds of bioreactor were used, which are ambr15, 3L bench-top reactor, and 200L bioreactor.
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